Sebelisa Mokhoa le Litlhokomelo tsa Freezing Tube
Litekong tsa microbiological, hangata ho sebelisoa sesebelisoa se le seng sa liteko, ke hore, cryopreservation tube.Leha ho le joalo, ka lebaka la ho rarahana ha bona ho fapaneng, liphello li fapana haholo.Ka lebaka lena, hajoale, lilaboratori tse ngata tsa Chaena li etsa li-tubes tsa ho boloka libaktheria ka botsona, tse sa eketsang matla a mosebetsi feela, empa hape ka lebaka la mefokolo ea maemo a fapaneng, phello ea ho boloka libaktheria ha e khotsofatse kamehla.
Ka hona, hoa hlokahala ho tseba mokhoa oa ts'ebeliso le litemoso tse ling tsa cryopreservation tube, e le hore u ka bapala karolo e kholo.
1.Mokhoa oa kopo
1).Ha u sebelisa cryopreservation tube ho boloka lisampole, ho hlokahala ka tieo ho kenya tšupu ea cryopreservation ka har'a mouoane oa naetrojene ea metsi kapa sehatsetsing bakeng sa polokelo.Haeba tube ea cryopreservation e bolokoa ka naetrojene ea metsi, ho na le monyetla o itseng oa hore naetrojene ea metsi e tla kenella ka har'a tube ea cryopreservation.Nakong ea ho hlaphoheloa, khase ea naetrojene e nang le metsi e tla lebisa ho se leka-lekaneng ha khatello ea ka hare le ea ka ntle, e leng ntho e ka 'nang ea etsa hore tube ea cryopreservation e phatlohe,' me e na le likotsi tsa likokoana-hloko.
2).Sebetsa cryopreservation tube ho tsosolosa, le ho sebelisa lisebelisoa tsa tšireletso ho pholletsa le ts'ebetso.Ho khothalletsoa ho apara liaparo tsa laboratori, liatlana tsa k'hothone le ho sebetsa bencheng e sireletsehileng ea laboratori.Haeba ho khonahala, ka kopo roala likhalase kapa tšireletso ea sefahleho.Kaha mocheso oa ka tlung lehlabula o tla ba holimo ho feta oa mariha, ka kopo ela hloko.
3).Nakong ea polokelo ea lisele tsa cryopreserved, mocheso o batang oa li-tubes tsa cryopreserved o tlameha ho tšoana.Ho qhoqhoa ho sa lekaneng ho tla lebisa ho jeme ea leqhoa, e tla thibela phetiso ea mocheso oa metsi mahlakoreng ka bobeli, kahoo e hlahise khatello e phahameng e kotsi le ho baka tšenyo ho tube e batang.
4).Palo ea lisampole tse hatselitsoeng ha ea lokela ho feta palo e kholo ea ho sebetsa e hlokoang ke tube e hatselitsoeng.
2. Litaba tse hlokang tlhokomelo
1).Sebaka sa polokelo ea li-tube tse batang
Li-tubes tsa cryopreservation tse sa sebelisoeng li ka bolokoa mocheso oa kamore kapa 2-8 ℃ bakeng sa likhoeli tse 12;The inoculated cryopreservation tube e ka bolokoa ho - 20 ℃ mme e na le phello e ntle ea ho boloka khatello nakong ea likhoeli tse 12;The ente e cryopreservation tube e ka bolokoa ho - 80 ℃, 'me mofuta ona o ka bolokoa hantle nakong ea likhoeli tse 24.
2).Nako ea ho boloka li-tubes tse batang
Li-tubes tsa cryopreservation tse sa sebelisoeng li ka bolokoa mocheso oa kamore kapa 2-8 ℃;Thepa e kentsoeng ea cryopreservation e tla bolokoa ho -20 ℃ kapa - 80 ℃.
3).Mehato ea ts'ebetso ea tube ea leqhoa
Nka litloaelo tse ncha ho tsoa litsong tse hloekileng tsa baktheria ho lokisa ho emisoa ha baktheria ka turbidity e ka bang 3-4 ea McDonnell's ratio bakeng sa ente le strain preservation tube;Tiisa tube ea ho boloka 'me u e khutlisetse morao le pele ka makhetlo a 4-5 ho etsa hore baktheria e be emulsified, ntle le ho potoloha;Kenya tube ea ho boloka ka sehatsetsing hore e bolokehe (- 20 ℃ - 70 ℃
Nako ea poso: Nov-25-2022