1. Lisebelisoa tse tloaelehileng
1. Thepa ka kamoreng ea ho lokisetsa
Mokelikeli o le mong oa metsi a hloekisitsoeng, sejana sa metsi se nang le metsi a mabeli, tanka ea asiti, ontong, cooker ea khatello, khabinete ea polokelo (ho boloka thepa e sa hloekisoang), khabinete ea polokelo (ho boloka thepa e hloekisitsoeng), tafole ea ho paka.Thepa e ka kamoreng ea ho lokisa tharollo: tekanyo ea torsion le tekanyo ea elektronike (moriana oa boima), PH meter (ho lekanya boleng ba PH ea tharollo ea setso), magnetic stirrer (ho lokisa phaposi ea tharollo ho hlohlelletsa tharollo).
2. Thepa ea kamore ea setso
Tanka ea naetrojene ea metsi, khabinete ea polokelo (ho boloka li-sundries), lebone la fluorescent le lebone la ultraviolet, sistimi e hloekisang moea, sehatsetsing sa mocheso o tlase (- 80 ℃), moea o futhumatsang moea, moqomo oa carbon dioxide, tafole e ka thoko (litlaleho tsa tlhahlobo ea ho ngola).
3. Thepa e tlamehang ho behoa ka phaposing e sa hloekang
Centrifuge (ho bokella lisele), ultra-clean worktable, inverted microscope, CO2 incubator (setso sa incubating), bate ea metsi, mochini o bolaeang likokoana-hloko o nang le oksijene e meraro le mochini oa sterilization, 4 ℃ sehatsetsi (ho beha serum le tharollo ea setso).
2. Ts'ebetso ea Aseptic
(1) Ho hloekisoa ha kamore e sa hloekang
1. Hloekisa kamore e sa hloekang kamehla: hang ka beke, sebelisa metsi a pompo pele ho phunya fatše, ho hlakola tafole le ho hloekisa tafole ea ho sebetsa, ebe u sebelisa 3 ‰ lysol kapa bromogeramine kapa 0.5% peracetic acid ho hlakola.
2. Sterilization ea CO2 incubator (incubator): pele o hlakola ka 3 ‰ bromogeramine, ebe o hlakola ka 75% ea joala kapa 0.5% peracetic acid, ebe o khantša lebone la ultraviolet.
3. Ho thibela pelehi pele ho teko: bulela lebone la ultraviolet, sterilizer ea oksijene e meraro le tsamaiso ea ho hloekisa moea ka metsotso e 20-30 ka ho latellana.
4. Sterilization ka mor'a teko: hlakola tafole ea ultra-hloekileng, tafole lehlakoreng le inverted microscope sethaleng ka 75% joala (3 ‰ bromogeramine).
Tokisetso ea sterilization ea basebetsi ba laboratori
1. Hlatsoa matsoho ka sesepa.
2. Apara liaparo tsa ho itšehla thajana, likatiba tsa ho itšehla thajana, limaske le li-slippers.
3. Hlakola matsoho ka bolo ea k'hothone ea joala ea 75%.
Pontšo ea ts'ebetso e nyopa
1. Libotlolo tsohle tsa joala, PBS, medium medium le trypsin tse tlisoang ka har'a benche ea mosebetsi e hloekileng haholo li lokela ho hlakoloa ka 75% ea joala ka ntle ho botlolo.
2. Sebetsa haufi le lelakabe la lebone la joala.
3. Lisebelisoa li tlameha ho hloekisoa pele li sebelisoa.
4. Lisebelisoa (tse kang li-bottle caps le droppers) tse ntseng li tsoela pele ho sebelisoa li lokela ho behoa sebakeng se phahameng, 'me li ntse li lokela ho futhumatsoa haholo nakong ea tšebeliso.
5. Ts'ebetso eohle e lokela ho ba haufi le lebone la joala, 'me ketso e lokela ho ba bonolo le e nepahetseng,' me ha ea lokela ho ama ka tšohanyetso.Haeba lehlokoa le sa khone ho ama tanka ea metsi a litšila.
6. Ha u lakatsa mefuta e fetang e 'meli ea mokelikeli, ela hloko ho nkela phala e monyang sebaka ho thibela tšilafalo.
Sheba khaolo e latelang bakeng sa ho bolaea likokoana-hloko tsa lisebelisoa.
Nako ea poso: Feb-01-2023