Litlhoko tsa pokello, polokelo le lipalangoang bakeng sa liteko tse tloaelehileng

Litlhoko tsa pokello, polokelo le lipalangoang bakeng sa liteko tse tloaelehileng

1. Ho bokella le ho boloka mehlala ea pathological:

☛Karolo e hatselitsoeng: Tlosa liboloko tse loketseng tsa linama ebe u li boloka ka har'a naetrojene e metsi;

☛Karohano ea parafine: Tlosa liboloko tse loketseng tsa 'mele 'me u li boloke ka har'a 4% ea paraformaldehyde;

☛Diselaete tsa disele: Diselaete tsa disele di ne di kentswe ka 4% paraformaldehyde metsotso e 30, mme tsa nkelwa sebaka ke PBS mme tsa qoetswa ho PBS mme tsa bolokwa ho 4°C.

2. Pokello le paballo ea mehlala ea baeloji ea limolek'hule:

☛Tishu e ncha: Khaola mohlala ebe u o boloka ka har'a metsi a naetrojene kapa -80°C sehatsetsing;

☛Mehlala ea Parafine: Boloka mocheso oa kamore;

☛Mohlala oa mali kaofela: Nka mali a lekaneng 'me u kenye EDTA kapa heparin anticoagulation ea pokello ea mali;

☛ lisampole tsa mokelikeli oa 'mele: centrifugation ea lebelo le phahameng ho bokella sediment;

☛Mehlala ea lisele: Lisele li tšeloa ka TRizol 'me li bolokoa ka har'a metsi a naetrojene kapa -80°C sehatsetsing.

3. Ho bokella le ho boloka mehlala ea liteko tsa protheine:

☛Tishu e ncha: Khaola mohlala ebe u o boloka ka har'a metsi a naetrojene kapa -80°C sehatsetsing;

☛Mohlala oa mali kaofela: Nka mali a lekaneng 'me u kenye EDTA kapa heparin anticoagulation ea pokello ea mali;

☛Mehlala ea lisele: Lisele li tšeloa ka botlalo ka motsoako oa cell lysis ebe li bolokoa ka har'a metsi a naetrojene kapa -80°C sehatsetsing.

4. Ho bokella le ho boloka ELISA, radioimmunoassay, le liteko tsa biochemical:

☛Serum (plasma) sampole: Nka mali a feletseng 'me u a kenye ka har'a tube ea procoagulation (anticoagulation tube), centrifuge ka 2500 rpm ka metsotso e ka bang 20, bokella supernatant,' me ue boloke ka metsi a nitrogen kapa ka sehatsetsing sa -80 ° C;

☛Sampole ea moroto: centrifuge sampole ka 2500 rpm ka metsotso e ka bang 20, 'me u e boloke ka har'a metsi a nitrogen kapa -80°C sehatsetsing;bua ka mokhoa ona bakeng sa mokelikeli oa thoracic le ascites, cerebrospinal fluid, le alveolar lavage fluid;

☛Lisampole tsa lisele: Ha u fumana likarolo tse patiloeng, centrifuge lisampole ka 2500 rpm ka metsotso e ka bang 20 ebe u li boloka ka har'a metsi a nitrogen kapa -80°C sehatsetsing;ha u lemoha likarolo tsa intracellular, Fokotsa ho emisoa ha sele ka PBS le ho qhoqhoa le ho qhibiliha khafetsa ho senya lisele le ho lokolla likarolo tsa intracellular.Centrifuge ka 2500 rpm bakeng sa metsotso e ka bang 20 'me u bokelle supernatant joalo ka holimo;

☛Mehlala ea maqeba: Ka mor'a ho seha mehlala, e lekanye 'me u e hatsele ka metsi a nitrogen kapa -80°C sehatsetsing hore se sebelisoe hamorao.

5. Pokello ea mohlala oa Metabolomics:

☛Sampole ea moroto: Centrifuge sampole ka 2500 rpm ka metsotso e ka bang 20 ebe u e boloka ka har'a metsi a nitrogen kapa -80°C sehatsetsing;bua ka mokhoa ona bakeng sa thoracic le ascites mokelikeli, cerebrospinal fluid, alveolar lavage fluid, joalo-joalo;

☛Ka mora ho seha sampole ya dithishu, e lekanye ebe o e hatsetsa ka metsi a naetrojene kapa sehatsetsing sa -80°C bakeng sa tshebediso ya hamorao;