Mokhoa oa ho sebelisa cryopreservation tube ka saense le ka nepo
Tšebeliso ea cryopreservation tube ke saense, eseng trilogy e bonolo joalo ka ho bula tanka ea naetrojene ea metsi, ho e kenya ka har'a tube ea cryopreservation le ho koala tanka ea naetrojene e mokelikeli.Tšebeliso ea mahlale le e nepahetseng ea cryopreservation tubes e ka qoba tahlehelo ea lisampole le ho sireletsa polokeho ea bahlahlobi.
Tšebeliso ea cryopreservation tube ke saense, eseng trilogy e bonolo joalo ka ho bula tanka ea naetrojene ea metsi, ho e kenya ka har'a tube ea cryopreservation le ho koala tanka ea naetrojene e mokelikeli.Tšebeliso ea mahlale le e nepahetseng ea cryopreservation tubes e ka qoba tahlehelo ea lisampole le ho sireletsa polokeho ea bahlahlobi.
Leqhoa la leqhoa: mehato e hatselang
Hlatsoa lisele ka tharollo ea PBS e chesitsoeng pele, monya tharollo, 'me u koahele lisele ka tharollo e nang le trypsin le EDTA (mokelikeli o mosesaane oa mokelikeli o lekane, mme motsoako oa trypsin le EDTA o hloka ho khethoa ho latela mohala oa sele).
Eketsa lisele ho 37 ℃ metsotso e 3-5.
Ka mor'a hore lisele li arohane ho tloha ka tlaase, ho kenngoa ho felile, serum se nang le serum se eketsoa, 'me lisele li emisoa ka bonolo ka pipette.
Centrifuge the cell suspension (500 xg, 5 min) le ho emisa hape ka sehare se nang le serum.
Palo ea lisele.
Centrifuge the cell suspension (500 xg, 5 metsotso), tlosa supernatant, 'me u tsosolose lisele tse nang le serum e nang le serum ea molumo o loketseng.
Kopanya lisele le cryopreservation solution (60% medium, 20% fetal bovine serum, 20% DMSO) ka tekanyo ea 1:1 ea molumo, ebe u li fetisetsa ho Cryo STM cryopreservation tube.Boima ba lisele tse leqhoa ke 1-5 × 106 likotoana / ml.
Cryo e nang le lisele tsa STM cryopreservation tube e khothalletsoa hore e pholile fatše ka tekanyo ea - 1 K / min, 'me tube ea cryopreservation e ka kenngoa ka setshelo se nang le isopropanol ho -70 ℃.Haeba Cryo STM cryopreservation tube e boloka disampole tse ding, tse ka bewang ka kotloloho ho − 20 ℃, − 70 ℃ kapa mogato wa gase wa naetrotšene ya seela.Ho etsa bonnete ba hore sampole e hoamisitsoe ka mokhoa o ts'oanang, 4 mL le 5 mL Cryo The sTM cryopreservation tube e hloka ho behoa ka sehatsetsing ka − 20 ℃ ka bosiu bo le bong, ebe e fetisetsoa mokhahlelong oa khase oa − 70 ℃ kapa naetrojene ea metsi.
Ebe u fetisetsa Cryo.sTM cryopreservation tube ho tanka ea naetrojene e metsi.E le ho qoba tšilafalo (joalo ka mycoplasma) le lintlha tsa polokeho, ka kopo beha Cryo.sTM cryopreservation tube mokhahlelong oa khase oa naetrojene ea mokelikeli, eseng karolong ea mokelikeli.
U ka sebelisa tube ea cryopreservation joang ka saense le ka nepo?Khamphani ea rona e entsoe ka litsebi tse nang le semelo sa indasteri le boiphihlelo bo bongata ba mebaraka ho fana ka lihlahisoa bakeng sa lefapha la lipatlisiso tsa mahlale a bophelo le lits'ebeletso ho bafuputsi ba mahlale.Ha e khone feela ho fihlela litlhoko tse khethehileng tsa bareki ba R&D ka mefuta ea lihlahisoa le liphutheloana, empa hape e ka fihlela litlhoko tse felletseng tsa likhoebo tsa tlhahiso ka mekhahlelo eohle ho tloha ka tekanyo e nyane, e mahareng ho isa ho tlhahiso e kholo.
Nako ea poso: Nov-25-2022